Coding

Part:BBa_K3241000:Design

Designed by: Ruxiang Wang   Group: iGEM19_HUBU-WUHAN   (2019-10-15)
Revision as of 15:05, 15 October 2019 by ZERO-Wang (Talk | contribs) (→‎Source)


PhbA(Beta-ketothiolase)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1126
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 225
    Illegal NgoMIV site found at 310
    Illegal NgoMIV site found at 715
    Illegal NgoMIV site found at 844
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 141


Design Notes

atgaccgatgttgtcattgtctctgccgctcgtaccgccgttggtaaatttggtggcagcttggccaaaattccggctcctgaattgggcgccgttgtcattaaagccgctttggaacgtgctggtgttaaaccggaacaggtctctgaagttattatgggccaagtcttgaccgctggtagcggtcagaatcctgctcgtcaagccgctattaaagccggtttgccggctatggtccctgccatgaccattaataaagtttgtggctctggtttgaaagccgtcatgttggccgctaatgctattatggccggcgatgctgaaattgttgtcgctggtggccaggaaaatatgtctgccgctccgcatgttttgcctggcagccgtgatggttttcgcatgggcgatgccaaattggtcgataccatgattgttgatggtttgtgggatgtttataatcagtatcatatgggcatcaccgctgaaaatgtcgccaaagaatatggtattacccgcgaagcccaagatgaatttgctgttggtagccagaataaagccgaagccgctcaaaaagctggcaaatttgatgaagaaattgtcccggttttgattcctcagcgtaaaggtgatccggttgcctttaaaaccgatgaatttgtccgccaaggtgctaccttggattctatgagcggcttgaaacctgcttttgataaagccggcaccgttaccgccgctaatgcctctggtttgaatgatggcgccgctgccgttgtcgttatgagcgctgccaaagccaaagaattgggtttgaccccgttggctaccattaaatcttatgctaatgccggcgtcgatcctaaagttatgggcatgggtccggttcctgcttctaaacgtgccttgagccgcgctgaatggaccccgcaggatttggatttgatggaaattaatgaagcctttgctgcccaagctttggccgtccatcagcaaatgggttgggatacctctaaagtcaatgttaatggtggcgctattgccattggccatcctattggcgccagcggttgtcgtattttggttaccttgttgcatgaaatgaaacgtcgcgatgccaaaaaaggcttggctagcttgtgcattggtggcggtatgggtgtcgctttggccgttgaacgcaaataa


Source

This gene is originally from Ralstonia eutropha.GenScript synthesized the gene and optimized its codon for us. This gene was cloned in pUC57.

References