Coding
Part:BBa_K3096007
Designed by: Famke Baeuerle, Lina Widerspick, Patrick Mueller, Jakob Keck Group: iGEM19_Tuebingen (2019-10-14)
Revision as of 14:48, 15 October 2019 by Lina widerspick (Talk | contribs)
This is a part consisting of all cofactors necessary for the functionality of the CRISPR/Cas3 system. It works if utilised with BBa_K3096001 and the respective CRISPR arrays targeting the to-be-degraded sequences.
Usage & Biology
In the type I CISPR/Cas system the crRNA guides the surveillance complex Cascade ABCDE to complementary DNA sequence. The DNA is unwound, generating the so-called R-loop. The Cas3 helicase-nuclease protein attaches to this R-loop and unidirectionally degrades the DNA. Hereby, the large Cas3/Cascade complex pulls the substrate DNA towards itself, introducing single strand breaks.
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Categories
Parameters
| None |