Composite
Part:BBa_K3096040
Designed by: Patrick Mueller, Famke Baeuerle, Lina Widerspick, Jakob Keck Group: iGEM19_Tuebingen (2019-10-14)
Revision as of 14:42, 15 October 2019 by Lina widerspick (Talk | contribs)
A constitutively active promoter expresses Clts, a temperature sensitive cI repressor of the lambda phage. If the temperature is significantly below 37°C (body temperature), Clts is an active repressor for the cI lambda promoter. This promoter controls the transcription of a repressor protein, AraC. Therefore, if conditions are not 37°C, the AraC is not available, hence the pBAD promoter, controlled by AraC, is active and genes controlled by the pBAD promoter are transcribed. Accordingly, if the temperature is at 37°C, Clts is unstable and cannot repress the expression of AraC. AraC consequently inhibits the pBAD promoter and downstream genes are not expressed.
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