Coding

Part:BBa_K2984019

Designed by: Darius Rauch   Group: iGEM19_Humboldt_Berlin   (2019-10-15)
Revision as of 14:28, 15 October 2019 by Chlamy Dima (Talk | contribs)


L1c-Psad-YFP-RbcS2, Expression of YFP through light-inducible promoter

This Level 1 Construct is designed for comparison of locus dependent expression through fluorescence intensity measuerments. The Construct consist of the Psad promoter an coding sequence for the yellow fluorescent protein and the Rbcs2 terminator. The promoter is inducible by light.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1046
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1046
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1046
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1046
    Illegal NgoMIV site found at 1523
  • 1000
    COMPATIBLE WITH RFC[1000]


Usage and Biology

This construct is designed for the expression of the yellow fluorescent protein mVenus in C. reinhardtii.

Characterization

[edit]
Categories
//function/reporter/fluorescence
Parameters
chassisC. reinhardtii
emission528 nm
excitation515 nm