Coding
Part:BBa_K2984019
Designed by: Darius Rauch Group: iGEM19_Humboldt_Berlin (2019-10-15)
Revision as of 14:22, 15 October 2019 by Chlamy Dima (Talk | contribs)
L1c-Psad-YFP-RbcS2, Expression of YFP through light-inducible promoter
This Level 1 Construct is designed for comparison of locus dependent expression through fluorescence intensity measuerments. The Construct consist of the Psad promoter an coding sequence for the yellow fluorescent protein and the Rbcs2 terminator. The promoter is inducible by light.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1046
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1046
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 4
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1046
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1046
Illegal NgoMIV site found at 1523 - 1000COMPATIBLE WITH RFC[1000]
Usage and Biology
Characterization
[edit]
Categories
Parameters
//function/reporter/fluorescence
chassis | C. reinhardtii |
emission | 528 nm |
excitation | 515 nm |