Coding

Part:BBa_K3141000

Designed by: Keyi Huang   Group: iGEM19_BSC_United   (2019-10-15)
Revision as of 13:58, 15 October 2019 by Eva Huang (Talk | contribs)


spacer; increasing proinsulin secretion

Based on others' previous research, we synthesized and modified the spacer sequence to make it more compatible to our project. Spacer BBa_K3141000, a short peptide consisting of 7 amino acids, has been proved that it is able to significantly increase proinsulin expression in Bacillus subtilis and Pichia pastoris through quantitative characterization. When using this spacer, the gene of this spacer is introduced into the N-terminal of HPI gene;then the peptide is synthesized.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None