Reporter

Part:BBa_K3112050

Designed by: Cheng Chen   Group: iGEM19_Tianjin   (2019-10-15)
Revision as of 13:42, 15 October 2019 by Registry (Talk | contribs)

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mRFP reporter with weak constitutive promoter(J23115)

Compared with the original part(BBa_J04450),We replace the lac1 promoter that may leak with a member of a constituent promoter family (BBa_J23115). It has a weak expression intensity, but can still be clearly observed under fluorescence microscopy.

We hope that this part can be used in co-expression scenarios with some colored products, and its weak expression intensity will not affect the observation of its colored products.

In addition, two restriction sites EcoRI and BamHI were added to both ends of the whole part by PCR. It is for the convenience of other teams to carry out further cloning operations or add other constitutive promoters or terminators.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4
    Illegal NheI site found at 16
    Illegal NheI site found at 39
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4
    Illegal BamHI site found at 924
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4
    Illegal AgeI site found at 635
    Illegal AgeI site found at 747
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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