Coding

Part:BBa_K3195004:Design

Designed by: Shan Baihui   Group: iGEM19_SEU-Nanjing-China   (2019-10-14)
Revision as of 13:14, 15 October 2019 by Bread (Talk | contribs) (→‎Source)


Trx-His-EK tagged subtilisin-like protease


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 447
    Illegal XhoI site found at 1645
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 889
    Illegal AgeI site found at 1120
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 967


Design Notes

It is the coding sequence of subtilisin-like protease found in Branchiostoma. We add 6 his tag at its tail, besides we optimized its condons for better prokaryotic expression.

Source

subtilisin-like protease gene from Branchiostoma belcheri tsingtauense

References