Coding

Part:BBa_K3187026

Designed by: iGEM TU_Darmstadt 2019   Group: iGEM19_TU_Darmstadt   (2019-10-12)
Revision as of 11:46, 15 October 2019 by Franziska (Talk | contribs)


mCherry Fluorescence Protein

name coat protein with LPETGG in pET24
base pairs 705
molecular weight 26.6 kDa
origin synthetic, derived from Discosoma sp.
properties Red fluorescent, Ex λ: 587nm, Em λ: 610 nm


mCherry is a red fluorescent protein, which is derived from Discosoma sp.. It absorbs light with a wavelength of 587 nm and emits light with a wavelength of 610 nm. mCherry can be modified N- and C-terminal without a deficit in fluorescent.

<img class="img-fluid center"
                src="T--TU_Darmstadt--Registry_mCherry.jpg"
                style="max-width:50%"/>

Figure 1: Crystal structure of mCherry.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None