Composite

Part:BBa_K3111103:Design

Designed by: Matas Deveikis   Group: iGEM19_UCL   (2019-09-18)
Revision as of 11:27, 15 October 2019 by Matas deveikis (Talk | contribs) (→‎Source)

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T. maritima encapsulin (6-His) with a StrepII-tag expressed under T7 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 183
    Illegal BglII site found at 598
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1013
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 532
    Illegal SapI.rc site found at 563


Design Notes

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Source

Encapsulin was isolated from Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099). Sequence was taken from BBa_K2686002 (without the stop codon as that would disable fusion on the C-terminus).

References

Moon, H., Lee, J., Min, J. and Kang, S. (2014). Developing Genetically Engineered Encapsulin Protein Cage Nanoparticles as a Targeted Delivery Nanoplatform. Biomacromolecules, 15(10), pp.3794-3801.