Coding
YFP B5-B5

Part:BBa_K2984020

Designed by: Darius Rauch   Group: iGEM19_Humboldt_Berlin   (2019-10-15)
Revision as of 10:57, 15 October 2019 by Registry (Talk | contribs)

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YFP - Yellow fluorescent protein for detection B5-B5

This Level 1 Construct is designed for comparrison of locus dependent expression through fluorescence intensity measuerments. The Construct consist of the Psad Promotor an coding sequence for the yellow fluorecent protein and the Rbcs2 terminator. The Protmer seems to be inducable by light.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 220
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 220
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 220
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 220
    Illegal NgoMIV site found at 697
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None