Translational_Unit

Part:BBa_K2999000

Designed by: Jinshui Lin   Group: iGEM19_YAU-China   (2019-10-14)
Revision as of 10:43, 15 October 2019 by Marxin (Talk | contribs) (→‎Usage and Biology)


RBS+PelA

This part is mainly used to encode the extracellular polysaccharide hydrolase PelA of Pseudomonas aeruginosa PAO1 biofilm and its hydrolysis. The first 100 bp of the sequence is its own RBS region.


Usage and Biology

Please click:BBa_K2999003 This part is useful as a reporter.

Uppsala2013 chromoproteins.JPG

iGEM13_Uppsala: Expression of chromoproteins. The images above show E coli constitutively expressing meffRed BBa_K1033922, aeBlue BBa_K1033929, tsPurple BBa_K1033905, asPink (asCP) BBa_K1033927, fwYellow BBa_K1033910, meffBlue BBa_K1033902, meffRed BBa_K1033922 and scOrange BBa_K1033913.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 274
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2427
    Illegal BamHI site found at 1410
    Illegal BamHI site found at 1575
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 191
    Illegal NgoMIV site found at 215
    Illegal NgoMIV site found at 236
    Illegal NgoMIV site found at 337
    Illegal NgoMIV site found at 563
    Illegal NgoMIV site found at 1271
    Illegal NgoMIV site found at 1320
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1157
    Illegal BsaI site found at 1783
    Illegal BsaI.rc site found at 1358


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Categories
Parameters
None