Composite

Part:BBa_K3096046

Designed by: Patrick Mueller, Famke Baeuerle, Jakob Keck, Lina Widerspick   Group: iGEM19_Tuebingen   (2019-10-15)
Revision as of 09:30, 15 October 2019 by Registry (Talk | contribs)

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Fatty acid responsive repression system

The intake of a fatty Acid meal increases the fatty Acid availability within the Body and therefore, their Metabolite Acyl-CoA will increase. Long chain Acyl-CoA will bind constitutively expressed FadR and hence inhibits ist activity. FadR is the repressor of the promoter pFad, which regulates the Expression of the LsrR repressor, which itself represses the pLsrR promoter. If there are no fatty acids available FadR is active inhibiting the pFad. No LsrR will be transcribed and genes under the Control of the pLsrR are active.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 902
    Illegal NheI site found at 925
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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