Composite
Part:BBa_K3096046
Designed by: Patrick Mueller, Famke Baeuerle, Jakob Keck, Lina Widerspick Group: iGEM19_Tuebingen (2019-10-15)
Fatty acid responsive repression system
The intake of a fatty Acid meal increases the fatty Acid availability within the Body and therefore, their Metabolite Acyl-CoA will increase. Long chain Acyl-CoA will bind constitutively expressed FadR and hence inhibits ist activity. FadR is the repressor of the promoter pFad, which regulates the Expression of the LsrR repressor, which itself represses the pLsrR promoter. If there are no fatty acids available FadR is active inhibiting the pFad. No LsrR will be transcribed and genes under the Control of the pLsrR are active.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 902
Illegal NheI site found at 925 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |