Coding

Part:BBa_K3317002:Design

Designed by: Erick Hernandez, Abigail Torres and Shakti Delgadillo   Group: iGEM19_UANL   (2019-10-15)
Revision as of 09:27, 15 October 2019 by Erick419 (Talk | contribs) (→‎References)


Protocatechuate decarboxylase (aroY) from Klebsiella pneumonia subsp.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 55
    Illegal AgeI site found at 381
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1045


Design Notes

sequence optimized to e. coli BL21, the ecoRI, XbaI, SpeI and PstI sites were avoided, a signal peptide () was added at its N terminal end with a flexible G4S linker


Source

ACCESSION BAH20873

References

Wu, W., Dutta, T., Varman, A. M., Eudes, A., Manalansan, B., Loqué, D., & Singh, S. (2017). Lignin valorization: Two hybrid biochemical routes for the conversion of polymeric lignin into value-added chemicals. Scientific reports, 7(1), 8420.