Part:BBa_K2936007
LacZ alpha
An alpha peptide chain encoded by a gene LacZ
Usage and Biology
The LacZ alpha gene is one of the structural genes present on the Lac Operon in Escherichia coli bacteria . When transcribed by RNA polymerase , the LacZ alpha gene codes for β-galactosidase , an enzyme responsible for the hydrolysis of β-galactosides (e.g. 2-Nitrophenyl β-D-galactopyranoside (ONPG) )
Characterize
LacZ alpha gene encodes the β-galactosidase, which could react with 2-Nitrophenyl β-D-galactopyranoside (ONPG) to generate orange color.
Mechanism:
Experimental Results
Form 1 The absorbance of different reaction solution at the wavelength of350 nm
< img src="https://2019.igem.org/wiki/images/5/5a/T--ZJUT-China--igem-part07form.png" alt=""width="600px">
Figure 1 The ration of ONPG is 1:2,1:4,1:8,1:10,1:15,1:20 from the left to right.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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