Regulatory

Part:BBa_K3031015:Design

Designed by: David Doyle   Group: iGEM19_SUIS_Shanghai   (2019-10-15)
Revision as of 06:55, 15 October 2019 by Registry (Talk | contribs)

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LuxI with FUR box located in the -10 region


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Insertion of the FUR box was informed by the report by Chu et al., (2015). This construct performed the best in experiments testing different constructs (as seen below) in low iron environments.


Source

BioBrick sequences.

References