Coding

Part:BBa_K3140004:Design

Designed by: Fahad Ali   Group: iGEM19_Sydney_Australia   (2019-10-12)
Revision as of 06:43, 15 October 2019 by Fahad3li (Talk | contribs) (→‎References)

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PsiH (codon optimised) - Cytochrome P450 monooxygenase from Psilocybe cubensis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part was intended for use in pUS381 for initial analysis of protein expression and final expression. To achieve this, the part was ordered in a gBlock flanked by BamHI and XbaI recognition sequences to enable traditional restriction cloning into pUS381, along with the consensus Shine-Dalgarno sequence as an RBS upstream of the coding sequence.

Source

The native sequence for the putative cytochrome P450 monooxygenase PsiH (Psilocybe cubensis) was obtained from NCBI: GenBank accession MF000993.1

References

1. Fricke, J., Blei, F. & Hoffmeister, D. Enzymatic Synthesis of Psilocybin. Angew Chem Int Ed Engl 56, 12352-12355 (2017).