Part:BBa_K3071024
Reporter construct in response to diffusible signal factor (DSF) with sequencing part
This composite part is the reporter construct that under the regulation of CBS I & II, which could be activated by pspF TAD-Clp upon DSF ligand binding. The upstream elements RpfC/RpfG (BBa_K3071022) is responsible for the sensing of diffusible signal factor (DSF) from Xanthomonas campestris pv. camperstris and signal transduction through phosphorylation. The co-activator psp TAD-Clp and sigma 54 (BBa_K3071023) are responsible for the activation of this composite part in response to the DSF binding.
In terms of gene structure, the -24 and -12 region is for the sigma 54-factor binding, with the -60 IHF interaction region and -129 to -89 UAS sequence I and II. The UAS sequence is replaced with the CBS I and CBS II UAS in gumB operon of Xanthomonas campestris pv. camperstris, to create a new synthetic promotor (BBa_K3071014). This promotor is conjugated with the eforRED with RBS (BBa_K1073023) for protein expression and give red chromoprotein signal.
In our rt-qPCR data, we demonstrate that the synthetic promotor could be function properly upon the treatment of azathioprine and DSF, with significant increase in eforRED RNA expression.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 105
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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