Coding

Part:BBa_K3021006

Designed by: Jeremy Hu   Group: iGEM19_PuiChing_Macau   (2019-10-14)
Revision as of 00:42, 15 October 2019 by Registry (Talk | contribs)

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pilA-Laccase1326-HisTag

This is a new composite part designed to allow Laccase secretion from our engineered cyanobacteria through embedded the secretion peptide, pilA1. Laccase is an important enzyme, which would degrade different chemicals. The Laccase sequence in this part is based on the laccase1326 cDNA sequence from previous study, which was shown to have great tolerant to different harsh conditions commonly found in polluted water. Secretion of heterologous proteins into cell culture medium offers significant advantages for downstream processing over production. pilA1 is the commonly know with its effect on the cyanobacteria secretion.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1014
    Illegal XhoI site found at 234
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 869


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