Coding

Part:BBa_K3190103:Design

Designed by: Ojas Tulsyan   Group: iGEM19_UCopenhagen   (2019-10-13)
Revision as of 20:40, 14 October 2019 by IbenNikolajsen (Talk | contribs)


G protein-coupled estrogen receptor (GPER) CDS with Linker-superfolder GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 750
    Illegal SapI.rc site found at 1162


Design Notes

The coding sequence of the GPER was fused with the nucleotides for the linker and superfolded GFP in the C-terminus (GPER-Li-sfGFP) to carry out localisation assay and characterise the expression and proper alignment of the receptor in the intercellular organelles.

The linker was added in the primer design.

Linker sequence: GGAGGTGGCTCTGGTGGTGGATCA


Source

Homo sapiens

References