Composite

Part:BBa_K3110033

Designed by: Mrugank Dake   Group: iGEM19_IISER_Tirupati   (2019-10-14)
Revision as of 20:08, 14 October 2019 by Registry (Talk | contribs)

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Strong Promoter Weak RBS lldP+lldR+lldD

L-Lactate Dehydrogenase (lldD) and L-Lactate Permease (lldP) and the regulator element (lldR) under the control of a strong promoter and a weak RBS.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 327
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3649
    Illegal AgeI site found at 463
    Illegal AgeI site found at 926
    Illegal AgeI site found at 1364
    Illegal AgeI site found at 1523
    Illegal AgeI site found at 1535
    Illegal AgeI site found at 2281
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 3086


[edit]
Categories
Parameters
None