Coding
EpsE

Part:BBa_K143032:Design

Designed by: Chris Hirst   Group: iGEM08_Imperial_College   (2008-09-18)
Revision as of 17:38, 18 September 2008 by Chris (Talk | contribs)

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EpsE Molecular Clutch Gene of B. subtilis



Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 762
    Illegal AgeI site found at 512
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The epsE(aka yveO) sequence was located in the B. subtilis chromosome#1 and the PstI restriction site removed before synthesis by GeneArt

Source

The epsE gene sequence was taken from the B. subtilis chromosome and was synthesised by GeneArt.

References

<biblio>

  1. 1 pmid=9384377

</biblio>