Coding

Part:BBa_K3096007

Designed by: Famke Baeuerle, Lina Widerspick, Patrick Mueller, Jakob Keck   Group: iGEM19_Tuebingen   (2019-10-14)
Revision as of 19:33, 14 October 2019 by JKe (Talk | contribs)

In the type I CISPR/Cas system the crRNA guides the surveillance complex Cascade ABCDE to complementary DNA sequence. The DNA is unwound, generating the so-called R-loop. The Cas3 helicase-nuclease protein attaches to this R-loop and unidirectionally degrades the DNA. Hereby, the large Cas3/Cascade complex pulls the substrate DNA towards itself, introducing single strand breaks.

[edit]
Categories
Parameters
None