Composite

Part:BBa_K3110030:Design

Designed by: Mrugank Dake   Group: iGEM19_IISER_Tirupati   (2019-10-14)
Revision as of 18:40, 14 October 2019 by Registry (Talk | contribs)

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Weak Promoter Weak RBS lldP+lldD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 327
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2857
    Illegal AgeI site found at 463
    Illegal AgeI site found at 926
    Illegal AgeI site found at 1364
    Illegal AgeI site found at 1523
    Illegal AgeI site found at 1535
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2294


Design Notes

lldD was codon optimized to meet synthesis requirements and to remove the illegal EcoRI site present in the genomic lldD.



Source

lldD was Synthesized from Twist Bioscience and was joined to lldP using SOEing (Spliced Overlap Extension) PCR. This was further joined to Promoter+RBS and Terminator using SOEing (Spliced Overlap Extension) PCR.


References