Part:BBa_K2922039
The colicin-E1 operon under pBAD (Arabinose promoter) control with an amajLime chromoprotein reporte
Summary
This is a composite part consist of an arabinose promoter (BBa_K206000), CDS of colicin-E1 (BBa_K2922024), CDS of colicin-E1 immunity protein (BBa_K2922025), CDS of kil protein (BBa_K2922026) and the amajLime chromoprotein reporter (BBa_K1033914) Each CDS has an RBS (BBa_B0034) behind. Pbad promoter could be induced by arabinose in Escherichia coli BL21 strain and then express all proteins mentioned. E coli that can`t express colicin-N immunity protein would be killed by colicin-E1, colicin-E1 immunity protein is used to protect itself from attack of extracellular colicin-E1 and kil protein helps the release of colicin-E1. The amajLime chromoprotein reporter is used to present the growth curve specifically of strain which contains this part. This part is constructed in the aim of achieve our "aggressive" design.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 125
Illegal NheI site found at 2360
Illegal NheI site found at 2383 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 65
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 642
Illegal AgeI site found at 2000
Illegal AgeI site found at 2057
Illegal AgeI site found at 2189 - 1000COMPATIBLE WITH RFC[1000]
| None |