Translational_Unit

Part:BBa_K3071023:Design

Designed by: Leung Hei Man   Group: iGEM19_Hong_Kong-CUHK   (2019-10-14)
Revision as of 17:06, 14 October 2019 by Registry (Talk | contribs)

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Co-activator construct for activating synthetic clp-regulated pspA promoter with sequencing part


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 953
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 956
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 953
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 953
    Illegal NgoMIV site found at 1031
    Illegal AgeI site found at 136
    Illegal AgeI site found at 164
    Illegal AgeI site found at 302
    Illegal AgeI site found at 2509
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Condon optimization for E. coli expression; Two sequencing primers flanked the whole construct for clone screening


Source

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References