Translational_Unit
Part:BBa_K3071023:Design
Designed by: Leung Hei Man Group: iGEM19_Hong_Kong-CUHK (2019-10-14)
Co-activator construct for activating synthetic clp-regulated pspA promoter with sequencing part
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 953
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 956
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 953
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 953
Illegal NgoMIV site found at 1031
Illegal AgeI site found at 136
Illegal AgeI site found at 164
Illegal AgeI site found at 302
Illegal AgeI site found at 2509 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Condon optimization for E. coli expression; Two sequencing primers flanked the whole construct for clone screening
Source
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