Composite

Part:BBa_K3110024

Designed by: Mrugank Dake   Group: iGEM19_IISER_Tirupati   (2019-10-14)
Revision as of 17:02, 14 October 2019 by Registry (Talk | contribs)

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Strong Promoter Weak RBS lldP+lldD

L-Lactate Dehydrogenase (lldD) and L-Lactate Permease (lldP) under the control of a strong promoter and a weak RBS.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 327
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2863
    Illegal AgeI site found at 463
    Illegal AgeI site found at 926
    Illegal AgeI site found at 1364
    Illegal AgeI site found at 1523
    Illegal AgeI site found at 1535
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2300


[edit]
Categories
Parameters
None