Regulatory
P43

Part:BBa_K143013:Design

Designed by: James Chappell   Group: iGEM08_Imperial_College   (2008-09-11)
Revision as of 13:06, 18 September 2008 by Jec105 (Talk | contribs) (→‎Source)

Promoter 43 a constitutive promoter for B. subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The biobrick part was designed to include the binding sites for both the sigma factor A and B. In addition the biobrick standard was applied to the promoter P43 sequence.


Source

The part was designed using the sequence from the B.subtilis genome and from previously published papers 1. This sequence was then synthesised by Geneart.

References

<biblio>

  1. 1 pmid=6330116

</biblio>