Regulatory
Pveg

Part:BBa_K143012

Designed by: James Chappell   Group: iGEM08_Imperial_College   (2008-09-11)
Revision as of 12:55, 18 September 2008 by Jec105 (Talk | contribs)

Promoter veg a constitutive promoter for B. subtilis

Pveg is a constitutive promoter that constitutively expresses the P43 protein in B. subtilis. Pveg contains binding sites for the B. sutbilis major sigma factor 1. Pveg in B. subtilis utilises two binding sites to cause high expression of genes #2, however this Pveg is lacking the upstream site to give a medium level of gene expression. It has been noted that the sporulation master regulatoion factor spoOA interacts with Pveg though it is not known how #3. The context with which we used the promoter Pveg is as a Polymerase Per Second (PoPS) generator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



References

<biblio>

  1. 1 pmid=6181373
  2. 2 pmid=6820069
  3. 3 pmid=14651647

</biblio>


[edit]
Categories
//rnap/prokaryote/subtilis/sigmaA
//direction/forward
//chassis/prokaryote/bsubtilis
//promoter
//regulation/constitutive
Parameters
negative_regulators
positive_regulators