Part:BBa_K769001
PompC-RBS-GFP-Double terminator
When OmpC promotor is activated by phosphorylated OmpR, GFP is expressed.
Usage and Biology
Characterization by team IGEM19_UI_Indonesia
Highest expression of GFP in TOP10 E. coli strain
Expression of GFP in TOP10 showed a sharp increase at 2.5 mg/20mL NaCl concentration compared to 2.0 mg/20mL concentration. This is contradicting our thinking that TOP10 will have a lower expression compared to BL21 because BL21 is a protein expression bacteria. A possible reason for this phenomenon is that there are differences in ompC promoter regulation in different bacterial strains. According to a characterization done by SDU-Denmark, it is found that OmpR-regulated promoter showed a high leaky expression in high copy vectors (e.g. TOP10) compared to low copy vectors (e.g. BL21) confirming our findings in our characterization. From the results, DH5a then BL21 showed a gradual increase in expression with increasing NaCl concentrations in LB medium. This indicates that DH5a and BL21 has a much more efficient control of gene expression with the ompC promoter.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
| None |