Regulatory

Part:BBa_J100034

Designed by: Margaret Stebbins   Group: Campbell M Lab   (2011-09-01)
Revision as of 15:39, 14 October 2019 by Eva Huang (Talk | contribs) (→‎Promoters and Their Primers)

groE promoter

The default state of this promoter is off and can be induced by heat. It is 44 base pairs long and is located 72 bases upstream of the start site of groE. The groE gene is from E. coli.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Promoters and Their Primers

Several promoters with high expression level that are commonly used in Bacillus subtilis were screened to determine the most efficient ones for our experiments on the construction of biological parts. Three of such promoters, Groe (BBa J100034), SecA (BBa K1469002), P43 (BBa K208002), exist in the iGEM registry already.Here we only show the data of Groe(BBa_J100034).

The primer sequences for individual promoters used for our synthetic biology project are as follows:

groe-up CGCGGATCCCCAATACTGTTTTCTCAAATGGTATGTA BamHI

groe-down CGGGGTACCTGAAATAACCTCCTCAATAGTATGA KpnI


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Parameters
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