Part:BBa_K3112041

Chromosomal stabilization element group example 2

Our chromosome stabilizing elements consist of two parts: centromeres and tag for screening.

This is our second example of testing chromosome stabilizing elements.The insertion site of this element is about 2K away from the centromere of Saccharomyces cerevisiae.This is also an another attempt to insert new centromere directly into the peripheral heterochromatin region. The aim of this attempt is to ensure that the separation site of this chromosome in the fusion system is consistent with that before fusion without affecting the original cell if possible.

Before fusion, the whole set of elements will not have a great impact on the growth and development of cells, it only plays its role in the fusion system. It can stabilize this chromosome with stable elements when the other side of the fusion system is dominant.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 133
  Illegal PstI site found at 234
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 1346
  Illegal PstI site found at 234
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 136
  Illegal BglII site found at 1237
  Illegal BglII site found at 1297
  Illegal BglII site found at 1514
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 133
  Illegal PstI site found at 234
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 133
  Illegal PstI site found at 234
  
• 1000
  COMPATIBLE WITH RFC[1000]