Part:BBa_K3132018
8*suntag
Signals in many biological processes can be amplified by recruiting multiple copies of regulatory proteins to a site of action. Harnessing this principle, a novel protein scaffold, a repeating peptide array termed SunTag was developed, which can recruit multiple copies of an antibody-fusion protein. Suntag comes from an antibody-peptide pair linked with GCN4, which significantly amplifies the glowing signals of green fluorescent proteins. Suntag is essentially a set of molecular hooks that hook multiple copies of biologically active molecules onto protein scaffolds that target to some genes or molecules. Compared with the assembled molecules without these hooks, the biological activity of the molecules integrated with SunTag was significantly amplified. Here we hope that IL-15 linked with suntag can better mediated receptor dimerization. SunTag8×consists of a string of eight GCN4 peptide tags. Moreover, to determine the best amount of these peptide tags, we set different enzyme cutting sites between every 2 GCN4 single chains, after cutting with different restriction enzymes, we got the other three types of IL-15 proteins labeled with different lengths of suntags: IL-15-suntag6×, IL-15-suntag4×, IL-15-suntag2×.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 280
Illegal NheI site found at 583 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 595
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 73
Illegal SapI.rc site found at 223
Illegal SapI.rc site found at 373
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