Composite

Part:BBa_K2921350

Designed by: Allison Kuo   Group: iGEM19_TAS_Taipei   (2019-10-14)
Revision as of 14:46, 14 October 2019 by Registry (Talk | contribs)

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Strong Promoter + RBS + 6xHIS + BacFerr + GS-linker + mRFP + Double Terminator

This construct constitutively expresses a colored metal-binding fusion protein: bacterioferritin (Basic part: BBa_K1438001) linked with mRFP (Basic part: BBa_E1010).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1154
    Illegal AgeI site found at 1266
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 222


[edit]
Categories
Parameters
None