Tag

Part:BBa_K3187018

Designed by: iGEM TU_Darmstadt 2019   Group: iGEM19_TU_Darmstadt   (2019-10-12)
Revision as of 14:15, 14 October 2019 by Lertu (Talk | contribs)


PolyG Tag (GGGG) for Sortase-mediated Ligation

  • Name: PolyG tag
  • bp: 12
  • Da: 300
  • Properties: second sequence for Sortase A

Introduction:

The enzyme Sortase A is able to form a peptide bond between a C-terminal LPXTG motif and a N-terminal poly glycine motif [1-3]. The DNA sequence below is coding for four glycines that can be used as the second recognition motif in a Sortase reaction.


Sequence:

GGCggaggcggt


References:

[1] Tsukiji, S. and Nagamune, T. (2009) Sortase-Mediated Ligation: A Gift from Gram-Positive Bacteria to Protein Engineering ChemBioChem

[2] Proft, T. (2010) Sortase-mediated protein ligation: an emerging biotechnology tool for protein modification and immobilisation

[3] Mao, H., Hart, S. A., Schink, A., and Pollok, B. A. (2004) Sortase-mediated protein ligation: a new method for protein engineering

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Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None