Part:BBa_K3132016
Generalized extracellular molecule sensor platform
The second generation CAR design, which fused a scFv, a CD8a hinge, a CD8 transmembrane domain, a 4-1BB intracellular domain and a CD3ζ chain in tandem was used in our project (Fig. 1). We designed two chimeric receptors that contain scFv derived from antibodies that recognize human EGFR and HER2. Cetuximab (anti-EGFR) and Trastuzumab (anti-HER2) were chosen because these antibodies have been found to be safe in patients when administered as targeted drugs. And therefore, these two receptors were named CTX.CAR and TTZ.CAR receptor, respectively. Unpaired cysteine 164 within the CD8a hinge region was replaced with a serine to increase CAR expression as reported previously.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1477
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2163
Illegal BglII site found at 2444
Illegal BamHI site found at 70
Illegal BamHI site found at 1770
Illegal XhoI site found at 64
Illegal XhoI site found at 991
Illegal XhoI site found at 2303 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2348
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 2234
Illegal BsaI.rc site found at 2598
Illegal SapI.rc site found at 1566
Illegal SapI.rc site found at 1581
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