Composite

Part:BBa_K3102027

Designed by: Ambre LELEU   Group: iGEM19_Ionis_Paris   (2019-10-07)
Revision as of 13:13, 14 October 2019 by ChloeDOIZELET (Talk | contribs)

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T7-RBS-LLDP-Ter

This T7-RBS-LLDP-ter composite is made of LLDP (L-lactate permease) part (BBa_K3102014) which is used to transports L-lactate across the membrane of E.coli.

This construct is enriched with T7 promoter, RBS and terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 324
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 460
    Illegal AgeI site found at 923
    Illegal AgeI site found at 1361
    Illegal AgeI site found at 1520
    Illegal AgeI site found at 1532
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None