Composite

Part:BBa_K3102026

Designed by: Ambre LELEU   Group: iGEM19_Ionis_Paris   (2019-10-07)
Revision as of 13:12, 14 October 2019 by ChloeDOIZELET (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


T7-RBS-LDHA-Ter

This T7-RBS-LDHA-ter composite is made of LDHA part (BBa_K3102006) which is used to catalyze the conversion of pyruvate in L-Lactate with concomitant inter-conversion of NADH and NAD+.

This construct is enriched with T7 promoter, RBS and terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 489
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 219


[edit]
Categories
Parameters
None