Coding

Part:BBa_K3015006

Designed by: Valentina Stuchlik   Group: iGEM19_BOKU-Vienna   (2019-10-14)
Revision as of 12:33, 14 October 2019 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


T7 RNA Polymerase (No BbsI/BpiI)

Since BbsI/BpiI can be a very important restriction enzyme for golden gate cloning in some labs, we decided to remove those recognition sites by codon swapping from the Part BBa_K14001 to improve the applicability.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None