Coding

Part:BBa_K3110000:Design

Designed by: Mrugank Dake   Group: iGEM19_IISER_Tirupati   (2019-10-13)
Revision as of 12:22, 14 October 2019 by Murgii (Talk | contribs) (→‎Source)


lldD (L-Lactate Dehydrogenase)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1149
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 586


Design Notes

The gene was codon-optimized for making it synthesis compatible and to remove the illegal restriction site EcoRI present in the genomic lldD.


Source

The sequence was synthesized by Twist Biosciences.

References