Reporter
miniSOG_TP
Part:BBa_K3111032:Design
Designed by: Matas Deveikis Group: iGEM19_UCL (2019-09-30)
Photosensitiser miniSOG fused to T. maritima encapsulin loading peptide
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 346
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Sequence was optimised for E. coli expression. Two illegal PstI sites removed from 44-Q and 95-Q (CAG to CAA). 15aa targeting peptide was chosen based on work by Cassidy-Amstutz et al, 2016.
Source
MiniSOG was derived from Arabidopsis thaliana. T. maritima targeting peptide taken from Cassidy-Amstutz et al, 2016.
References
Cassidy-Amstutz C, Oltrogge L, Going CC, Lee A, Teng P, Quintanilla D, et al. Identification of a Minimal Peptide Tag for in Vivo and in Vitro Loading of Encapsulin. Biochemistry. 2016 Jun 21;55(24):3461–