Coding

Part:BBa_K3279004:Design

Designed by: Shujie Liao   Group: iGEM19_CAU_China   (2019-10-14)
Revision as of 09:33, 14 October 2019 by JIAO (Talk | contribs) (→‎Design Notes)

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beta-carotene 3-hydroxylase (CrtZ)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 226
    Illegal PstI site found at 436
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 514
    Illegal PstI site found at 226
    Illegal PstI site found at 436
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 226
    Illegal PstI site found at 436
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 226
    Illegal PstI site found at 436
    Illegal AgeI site found at 211
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence was synthesized by Sangon Biotech and the codon of this part was optimized to increase its translation rate in E. coli.

Source

The original sequence comes from Pantoea ananatis but we have codon optimized it.

References