Part:BBa_K3268001
Lac-inducible generator of Lpp-OmpA(46-159)-mOR103-15 + His6 tag
This part contains a lac promoter and strong ribosome binding site for lac-inducible expression of the fusion protein of Lpp signal peptide, OmpA aa46-159, and mOR103-15 + His6 tag. This expression should display streptavidin on the cell surface of E. coli.
In order to display mOR103-15 on the surface of cell, we found that the Lpp′OmpA(46-159) hybrid protein can serve as an efficient targeting vehicle for localizing a variety soluble proteins onto the E. coli surface.We got the part BBa_J36850 which is combined with a lac promoter and strong ribosome binding site for lac-inducible expression of the fusion protein of Lpp signal peptide, OmpA aa46-159, and streptavidin wild-type + His6 tag from iGEM kit 2019.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1083
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1057
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1463
None |