Part:BBa_K2989001

SCP1 -HBsAg

The serine/threonine phosphatase (PSP) in the protein contains three major families: phosphorylated protein phosphoprotein esterases (PPPs), metal-dependent phosphatases (PPMs), and aspartate-based phosphatases. Among them, SCP1 belongs to the third family, an aspartate-based phosphatase. It is highly conserved from fruit flies to mammals. The sequence of the 31 to 55 position of SCP1 has a membrane localization effect in various cell lines, such as human embryonic kidney cell HEK293T, human colon cancer cell DLD1, and human breast cancer epithelial cell MCF-7. Therefore, we chose this sequence to localize the HsBAg on the surface of the tumor cells. Hepatitis B surface antigen (HBsAg) is an outer membrane protein of HBV encoded by the HBV S gene region. Hepatitis B surface antigen is the main immunogen of host protective immunity, which can stimulate the body to produce neutralizing antibodies, also known as protective antibodies HBsAb. It is generally believed that the appearance of HBs Ab marks the termination of HBV infection. Here, we chose a fragment of S.proteinas artificial new antigens，which contains CD4+ and CD8+ T cell epitopes. We can take it as 'a "Trojan Horse" antigen.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]