Composite

Part:BBa_K3052030

Designed by: Ma WenHe   Group: iGEM19_XJTU-CHINA   (2019-10-14)
Revision as of 08:26, 14 October 2019 by Registry (Talk | contribs)

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Light-fluoresce circuit (GFP&RFP)

This circuit uses a light control system wherein the central protein cph8 is a photosensitive protein that can response to red light and block the expression of downstream genes. The variables of this two-phases can be calculated by measuring the fluoresce intensity of sfGFP and mRFP.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 5032
    Illegal NheI site found at 5055
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2298
    Illegal BglII site found at 6592
    Illegal XhoI site found at 405
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 6145
    Illegal AgeI site found at 4801
    Illegal AgeI site found at 4913
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2504


[edit]
Categories
Parameters
None