Part:BBa_K2923002

Theophylline inactif aptazyme

An aptazyme is an aptamer-ribozyme combination with intrinsic RNA cleavage activity. this aptazyme is specific for the theophylline (ligand). Theophylline (1,3-dimethylxanthine) is a drug used in therapy for respiratory diseases and is structurally similar to theobromine and caffeine. It is composed of 3 different parts, a twister ribozyme module, a communication module, and an aptamer module. This sequence corresponds to inactive aptazyme: the ribozyme module is mutated and the aptazyme lost self-cleavage capability. iGEM Strasbourg 2019 used it as a negative control for the part: BBa_K2923001

Figure 1: Theophylline aptazyme secondary structure. The Shine-Dalgarno (SD) and the anti-SD are shown in light blue and grey, respectively. The inactivating mutation is highlighted in yellow. The cleavage site is indicated with a grey arrow. The figure is reproduced from [Stifel, J., Spöring, M., and Hartig, J.S. (2019). Expanding the toolbox of synthetic riboswitches with guanine-dependent aptazymes. Synthetic Biology 4

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]