Translational_Unit

Part:BBa_K3071022:Design

Designed by: Leung Hei Man   Group: iGEM19_Hong_Kong-CUHK   (2019-10-14)
Revision as of 07:01, 14 October 2019 by Registry (Talk | contribs)

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Recombinant Two-component regulatory system RpfG/RpfC construct with sequencing part


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 349
    Illegal NgoMIV site found at 2647
    Illegal AgeI site found at 1181
    Illegal AgeI site found at 1660
    Illegal AgeI site found at 1744
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 398
    Illegal BsaI.rc site found at 262


Design Notes

Condon optimization for E. coli expression; Correction of the stop codon in the original reported sequence; Two sequencing primers flanked the whole construct for clone screening


Source

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References