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Translational_Unit
Part:BBa_K3071022:Design
Designed by: Leung Hei Man Group: iGEM19_Hong_Kong-CUHK (2019-10-14)
Recombinant Two-component regulatory system RpfG/RpfC construct with sequencing part
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 349
Illegal NgoMIV site found at 2647
Illegal AgeI site found at 1181
Illegal AgeI site found at 1660
Illegal AgeI site found at 1744 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 398
Illegal BsaI.rc site found at 262
Design Notes
Condon optimization for E. coli expression; Correction of the stop codon in the original reported sequence; Two sequencing primers flanked the whole construct for clone screening
Source
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