Composite
Part:BBa_K3110002:Design
Designed by: Mrugank Dake Group: iGEM19_IISER_Tirupati (2019-10-14)
lldP+lldD
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 275
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2805
Illegal AgeI site found at 411
Illegal AgeI site found at 874
Illegal AgeI site found at 1312
Illegal AgeI site found at 1471
Illegal AgeI site found at 1483 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2242
Design Notes
lldD has an EcoRI site, which we couldn't eliminate and hence the part needs further improvement.
Source
This has been amplified from the genome.