Part:BBa_K3140000
PsiD - Tryptophan decarboxylase from Psilocybe cubensis
PsiD is a tryptophan decarboxylase, which catalyses the conversion of L-tryptophan to tryptamine.
Usage and Biology
PsiD is a native enzyme obtained from the Psilocybe cubensis, which is involved in the metabolic biosynthesis of psilocybin from tryptophan. It accepts both L-tryptophan and 4-hydroxy-L-tryptophan as substrates, producing tryptamine and 4-hydroxytryptamine, respectively. Heterologous expression of PsiD has been achieved in Escherichia coli <add strain> in a <expression system>.
[[https://static.igem.org/mediawiki/parts/f/f0/
[[Image:T--Sydney_Australia--PsiD_KEGG_rxn1.gif|thumb|900px|Fig. 1: Decarboxylation of L-tryptophan to tryptamine, mediated by PsiD. [Source: KEGG]]]
ADD:
- SDS-PAGE IMAGE
- LC/MS
- pUS250 and pET-28c(+)
Sequence and Features
| None |