Composite
IBR-G22
Part:BBa_K3254005:Design
Designed by: Yeqing Zong, YI REN Group: iGEM19_GENAS_China (2019-10-13)
Revision as of 04:04, 14 October 2019 by Zongyeqing (Talk | contribs)
TG1attB-BsaI site-terminator-TG1attP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 60
Illegal BsaI.rc site found at 48
Design Notes
This part sequence was tested in silico before synthesised to avoid potential promoter activity (see the website in references). A mutation (T to A) was made for avoiding an unexpected BsaI site.
Source
This part was totally synthesised by commerical company.
References
Chen, Y.J., et al., Characterization of 582 natural and synthetic terminators and quantification of their design constraints. Nat Methods, 2013. 10(7): p. 659-64. http://www.fruitfly.org/seq_tools/promoter.html